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No. 6050195) coated the plate and it absolutely was positioned inside of a BioTek Cytation3 Imaging Reader. Kinetic knowledge was acquired every single 2hrs for 24hrs utilizing GFP details acquisition. The gas period all over the acquisition of kinetic knowledge was 5% O2, 5% CO2 with the harmony of nitrogen managed by a BioTek CO2/O2 gas controlle
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